1DYS

Endoglucanase CEL6B from Humicola insolens

Summary for 1DYS

• Entry DOI: 10.2210/pdb1dys/pdb
• Descriptor: ENDOGLUCANASE (2 entities in total)
• Functional Keywords: cellulase, hydrolase, cellulose degradation, glycoside hydrolase family 6
• Biological source: HUMICOLA INSOLENS
• Total number of polymer chains: 2
• Total formula weight: 75571.66

Authors

Davies, G.J.,Brzozowski, A.M.,Dauter, M.,Varrot, A.,Schulein, M. (deposition date: 2000-02-08, release date: 2001-02-08, Last modification date: 2024-11-13)

Primary citation

Davies, G.J.,Brzozowski, A.M.,Dauter, M.,Varrot, A.,Schulein, M.
Structure and Function of Humicola Insolens Family 6 Cellulases: Structure of the Endoglucanase, Cel6B, at 1.6 A Resolution
Biochem.J., 348:201-, 2000

PubMed Abstract: Cellulases are traditionally classified as either endoglucanases or cellobiohydrolases on the basis of their respective catalytic activities on crystalline cellulose, which is generally hydrolysed more efficiently only by the cellobiohydrolases. On the basis of the Trichoderma reesei cellobiohydrolase II structure, it was proposed that the active-site tunnel of cellobiohydrolases permitted the processive hydrolysis of cellulose, whereas the corresponding endoglucanases would display open active-site clefts [Rouvinen, Bergfors, Teeri, Knowles and Jones (1990) Science 249, 380-386]. Glycoside hydrolase family 6 contains both cellobiohydrolases and endoglucanases. The structure of the catalytic core of the family 6 endoglucanase Cel6B from Humicola insolens has been solved by molecular replacement with the known T. reesei cellobiohydrolase II as the search model. Strangely, at the sequence level, this enzyme exhibits the highest sequence similarity to family 6 cellobiohydrolases and displays just one of the loop deletions traditionally associated with endoglucanases in this family. However, this enzyme shows no activity on crystalline substrates but a high activity on soluble substrates, which is typical of an endoglucanase. The three-dimensional structure reveals that the deletion of just a single loop of the active site, coupled with the resultant conformational change in a second 'cellobiohydrolase-specific' loop, peels open the active-site tunnel to reveal a substrate-binding groove.

PubMed: 10794732
DOI: 10.1042/BJ3480201

Experimental method

X-RAY DIFFRACTION (1.6 Å)