1DYS
Endoglucanase CEL6B from Humicola insolens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 109.917, 104.451, 53.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.18 * |
Rwork | 0.180 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TRICHODERMA REESEI CELLOBIOHYDROLASE II (T A JONES PERSONAL COMMUNICATION) |
RMSD bond length | 0.012 |
RMSD bond angle | 0.029 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.065 | 0.317 |
Number of reflections | 78038 | |
<I/σ(I)> | 17 | 3.6 |
Completeness [%] | 98.9 | 98 * |
Redundancy | 3.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | THE PROTEIN (20 MG/ML-1) WAS CRYSTALLISED FROM 30% (W/V) PEG 4000K IN 10MM TRIS-ACETATE BUFFER AT PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | reservoir | Tris-HCl | 0.1 (M) |