1DLR

METHOTREXATE-RESISTANT VARIANTS OF HUMAN DIHYDROFOLATE REDUCTASE WITH SUBSTITUTION OF LEUCINE 22: KINETICS, CRYSTALLOGRAPHY AND POTENTIAL AS SELECTABLE MARKERS

Summary for 1DLR

DescriptorDIHYDROFOLATE REDUCTASE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 6-(2,5-DIMETHOXY-BENZYL)-5-METHYL-PYRIDO[2,3-D]PYRIMIDINE-2,4-DIAMINE, ... (4 entities in total)
Functional Keywordsoxido-reductase, oxidoreductase
Biological sourceHomo sapiens (human)
Total number of polymer chains1
Total molecular weight22454.33
Authors
Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W. (deposition date: 1995-01-25, release date: 1995-04-20, Last modification date: 2011-07-13)
Primary citation
Lewis, W.S.,Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W.,Chunduru, S.K.,Spencer, H.T.,Appleman, J.R.,Blakley, R.L.
Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.
J.Biol.Chem., 270:5057-5064, 1995
PubMed: 7890613 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.270.10.5057
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliers2307.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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