1DLR

METHOTREXATE-RESISTANT VARIANTS OF HUMAN DIHYDROFOLATE REDUCTASE WITH SUBSTITUTION OF LEUCINE 22: KINETICS, CRYSTALLOGRAPHY AND POTENTIAL AS SELECTABLE MARKERS

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	87.002, 87.002, 76.728
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 2.300
R-factor	0.167
RMSD bond length	0.016
RMSD bond angle	0.051
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.000
Number of reflections	10046
Completeness [%]	67.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	NADPH		a molar excess of
2	1	drop	phosphate	0.1 (M)
3	1	drop	ammonium sulfate	62 (%)