1CQS

CRYSTAL STRUCTURE OF D103E MUTANT WITH EQUILENINEOF KSI IN PSEUDOMONAS PUTIDA

Summary for 1CQS

• Entry DOI: 10.2210/pdb1cqs/pdb
• Related: 1OPY
• Descriptor: PROTEIN : KETOSTEROID ISOMERASE, EQUILENIN (3 entities in total)
• Functional Keywords: ksi, equilenin, putida lbhb, isomerase
• Biological source: Pseudomonas putida
• Total number of polymer chains: 2
• Total formula weight: 29655.75

Authors

Choi, G.,Ha, N.C.,Kim, S.W.,Kim, D.H.,Park, S.,Oh, B.H.,Choi, K.Y. (deposition date: 1999-08-11, release date: 2003-06-17, Last modification date: 2024-02-07)

• Primary citation: Choi, G.,Ha, N.C.,Kim, S.W.,Kim, D.H.,Park, S.,Oh, B.H.,Choi, K.Y.; Asp-99 donates a hydrogen bond not to Tyr-14 but to the steroid directly in the catalytic mechanism of Delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B; Biochemistry, 39:903-909, 2000; PubMed: 10653633; DOI: 10.1021/bi991579k

Experimental method

X-RAY DIFFRACTION (1.9 Å)