1CQS
CRYSTAL STRUCTURE OF D103E MUTANT WITH EQUILENINEOF KSI IN PSEUDOMONAS PUTIDA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-01 |
Detector | MACSCIENCE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.257, 72.081, 51.335 |
Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.25900 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.790 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.046 * | 0.272 |
Number of reflections | 21961 | |
<I/σ(I)> | 16 | |
Completeness [%] | 86.6 | 75.1 |
Redundancy | 5.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 22 * | Sodium Acetate, Ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-Cl | 50 (mM) | pH7.0 |
3 | 1 | drop | beta-mercaptoethanol | 20 (mM) | |
4 | 1 | reservoir | ammonium acetate | 1.1 (M) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |