1CHP

SURPRISING LEADS FOR A CHOLERA TOXIN RECEPTOR BINDING ANTAGONIST; CRYSTALLOGRAPHIC STUDIES OF CTB MUTANTS

Summary for 1CHP

• Entry DOI: 10.2210/pdb1chp/pdb
• Descriptor: CHOLERA TOXIN B PENTAMER, CHLORIDE ION (3 entities in total)
• Functional Keywords: toxin
• Biological source: Vibrio cholerae
• Total number of polymer chains: 5
• Total formula weight: 58441.97

Authors

Merritt, E.A.,Hol, W.G.J. (deposition date: 1995-02-15, release date: 1996-03-08, Last modification date: 2024-11-20)

Primary citation

Merritt, E.A.,Sarfaty, S.,Chang, T.T.,Palmer, L.M.,Jobling, M.G.,Holmes, R.K.,Hol, W.G.
Surprising leads for a cholera toxin receptor-binding antagonist: crystallographic studies of CTB mutants.
Structure, 3:561-570, 1995

PubMed Abstract: Because agents which inhibit the receptor binding of cholera toxin constitute possible lead compounds for the structure-based design of anti-cholera drugs, detailed investigation of the toxin's receptor-binding site is of key importance. The substitution Gly-->Asp at residue 33 of the cholera toxin B subunit (CTB) has been reported to abolish receptor-binding ability. The substitution Arg35-->Asp has been reported to result in deficient assembly of the AB5 holotoxin. The molecular basis for these effects was not readily apparent from analysis of an earlier crystal structure of the wild-type toxin B pentamer in a complex with the receptor pentasaccharide.

PubMed: 8590017
DOI: 10.1016/S0969-2126(01)00190-3

Experimental method

X-RAY DIFFRACTION (2 Å)