1CD9

2:2 COMPLEX OF G-CSF WITH ITS RECEPTOR

Summary for 1CD9

• Entry DOI: 10.2210/pdb1cd9/pdb
• Descriptor: PROTEIN (GRANULOCYTE COLONY-STIMULATING FACTOR), PROTEIN (G-CSF RECEPTOR), 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: class1 cytokine, hematopoietic receptor, signal transduction, cytokine
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 86996.46

Authors

Aritomi, M.,Kunishima, N.,Okamoto, T.,Kuroki, R.,Ota, Y.,Morikawa, K. (deposition date: 1999-03-08, release date: 2000-03-08, Last modification date: 2024-11-20)

Primary citation

Aritomi, M.,Kunishima, N.,Okamoto, T.,Kuroki, R.,Ota, Y.,Morikawa, K.
Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme.
Nature, 401:713-717, 1999

PubMed Abstract: Granulocyte colony-stimulating factor (GCSF) is the principal growth factor regulating the maturation, proliferation and differentiation of the precursor cells of neutrophilic granulocytes and is used to treat neutropenia. GCSF is a member of the long-chain subtype of the class 1 cytokine superfamily, which includes growth hormone, erythropoietin, interleukin 6 and oncostatin M. Here we have determined the crystal structure of GCSF complexed to the BN-BC domains, the principal ligand-binding region of the GCSF receptor (GCSFR). The two receptor domains form a complex in a 2:2 ratio with the ligand, with a non-crystallographic pseudo-twofold axis through primarily the interdomain region and secondarily the BC domain. This structural view of a gp130-type receptor-ligand complex presents a new molecular basis for cytokine-receptor recognition.

PubMed: 10537111
DOI: 10.1038/44394

Experimental method

X-RAY DIFFRACTION (2.8 Å)