1CD9

2:2 COMPLEX OF G-CSF WITH ITS RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-15
Detector	RIGAKU
Spacegroup name	I 41 2 2
Unit cell lengths	125.467, 125.467, 372.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.800
Rwork	0.237
R-free	0.29800 *
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Rmerge	0.057
Total number of observations	169036 *
Number of reflections	35565
Completeness [%]	95.4
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		manuscript in preparation *