1BR6
RICIN A CHAIN (RECOMBINANT) COMPLEX WITH PTEROIC ACID
Summary for 1BR6
| Entry DOI | 10.2210/pdb1br6/pdb |
| Descriptor | PROTEIN (RICIN), PTEROIC ACID (3 entities in total) |
| Functional Keywords | glycosidase, hydrolase |
| Biological source | Ricinus communis (castor bean) |
| Total number of polymer chains | 1 |
| Total formula weight | 30380.24 |
| Authors | Hollis, T.,Yan, X.,Svinth, M.,Day, P.,Monzingo, A.F.,Milne, G.W.A.,Robertus, J.D. (deposition date: 1998-08-27, release date: 1998-09-02, Last modification date: 2023-08-09) |
| Primary citation | Yan, X.,Hollis, T.,Svinth, M.,Day, P.,Monzingo, A.F.,Milne, G.W.,Robertus, J.D. Structure-based identification of a ricin inhibitor. J.Mol.Biol., 266:1043-1049, 1997 Cited by PubMed Abstract: Ricin is a potent cytotoxin which has been used widely in the construction of therapeutic agents such as immunotoxins. Recently it has been used by governments and underground groups as a poison. There is interest in identifying and designing effective inhibitors of the ricin A chain (RTA). In this study computer-assisted searches indicated that pterins might bind in the RTA active site which normally recognizes a specific adenine base on rRNA. Kinetic assays showed that pteroic acid could inhibit RTA activity with an apparent Ki of 0.6 mM. A 2.3 A crystal structure of the complex revealed the mode of binding. The pterin ring displaces Tyr80 and binds in the adenine pocket making specific hydrogen bonds to active site residues. The benzoate moiety of pteroic acid binds on the opposite side of Tyr80 making van der Waals contact with the Tyr ring and forming a hydrogen bond with Asn78. Neopterin, a propane triol derivative of pterin, also binds to RTA as revealed by the X-ray structure of its complex with RTA. Neither pterin-6-carboxylic acid nor folic acid bind to the crystal or act as inhibitors. The models observed suggest alterations to the pterin moiety which may produce more potent and specific RTA inhibitors. PubMed: 9086280DOI: 10.1006/jmbi.1996.0865 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
Download full validation report
