1BR6
RICIN A CHAIN (RECOMBINANT) COMPLEX WITH PTEROIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-08-15 |
| Detector | SDMS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.992, 64.366, 49.442 |
| Unit cell angles | 90.00, 107.98, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.300 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 26.270 * |
| Data reduction software | UCSD-system |
| Data scaling software | UCSD-system |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.061 | 0.135 |
| Number of reflections | 10999 | |
| <I/σ(I)> | 11.3 | 4.2 |
| Completeness [%] | 96.0 | 78 |
| Redundancy | 6.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.9 | 4 * | Robertus, J.D., (1987) J. Biol. Chem., 262, 19. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2-2.5 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 75 (mM) | |
| 3 | 1 | reservoir | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) |
