1BJT

TOPOISOMERASE II RESIDUES 409-1201

1BJT の概要

• エントリーDOI: 10.2210/pdb1bjt/pdb
• 分子名称: TOPOISOMERASE II (2 entities in total)
• 機能のキーワード: topoisomerase, quaternary change, dna-binding, dna topology
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P06786
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 91810.60

構造登録者

Fass, D.,Bogden, C.E.,Berger, J.M. (登録日: 1998-06-29, 公開日: 1999-05-04, 最終更新日: 2024-02-07)

主引用文献

Fass, D.,Bogden, C.E.,Berger, J.M.
Quaternary changes in topoisomerase II may direct orthogonal movement of two DNA strands.
Nat.Struct.Biol., 6:322-326, 1999

PubMed Abstract: Type II DNA topoisomerases mediate the passage of one DNA duplex through a transient break in another, an event essential for chromosome segregation and cell viability. The active sites of the type II topoisomerase dimer associate covalently with the DNA break-points and must separate by at least the width of the second DNA duplex to accommodate transport. A new structure of the Saccharomyces cerevisiae topoisomerase II DNA-binding and cleavage core suggests that in addition to conformational changes in the DNA-opening platform, a dramatic reorganization of accessory domains may occur during catalysis. These conformational differences have implications for both the DNA-breaking and duplex-transport events in the topo II reaction mechanism, suggest a mechanism by which two distinct drug-resistance loci interact, and illustrate the scope of structural changes in the cycling of molecular machines.

PubMed: 10201398
DOI: 10.1038/7556

実験手法

X-RAY DIFFRACTION (2.5 Å)