1BI7

MECHANISM OF G1 CYCLIN DEPENDENT KINASE INHIBITION FROM THE STRUCTURE OF THE CDK6-P16INK4A TUMOR SUPPRESSOR COMPLEX

1BI7 の概要

• エントリーDOI: 10.2210/pdb1bi7/pdb
• 分子名称: CYCLIN-DEPENDENT KINASE 6, MULTIPLE TUMOR SUPPRESSOR (2 entities in total)
• 機能のキーワード: cyclin dependent kinase, cyclin dependent kinase inhibitory protein, cdk, ink4, cell cycle, multiple tumor suppressor, mts1, complex (kinase-anti-oncogene), complex (kinase-anti-oncogene) complex, complex (kinase/anti-oncogene)
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P42771
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53542.91

構造登録者

Russo, A.A.,Tong, L.,Lee, J.O.,Jeffrey, P.D.,Pavletich, N.P. (登録日: 1998-06-22, 公開日: 1999-01-13, 最終更新日: 2024-02-07)

主引用文献

Russo, A.A.,Tong, L.,Lee, J.O.,Jeffrey, P.D.,Pavletich, N.P.
Structural basis for inhibition of the cyclin-dependent kinase Cdk6 by the tumour suppressor p16INK4a.
Nature, 395:237-243, 1998

PubMed Abstract: The cyclin-dependent kinases 4 and 6 (Cdk4/6) that control the G1 phase of the cell cycle and their inhibitor, the p16INK4a tumour suppressor, have a central role in cell proliferation and in tumorigenesis. The structures of Cdk6 bound to p16INK4a and to the related p19INK4d reveal that the INK4 inhibitors bind next to the ATP-binding site of the catalytic cleft, opposite where the activating cyclin subunit binds. They prevent cyclin binding indirectly by causing structural changes that propagate to the cyclin-binding site. The INK4 inhibitors also distort the kinase catalytic cleft and interfere with ATP binding, which explains how they can inhibit the preassembled Cdk4/6-cyclin D complexes as well. Tumour-derived mutations in INK4a and Cdk4 map to interface contacts, solidifying the role of CDK binding and inhibition in the tumour suppressor activity of p16INK4a.

PubMed: 9751050
DOI: 10.1038/26155

実験手法

X-RAY DIFFRACTION (3.4 Å)