1AHJ

NITRILE HYDRATASE

Summary for 1AHJ

• Entry DOI: 10.2210/pdb1ahj/pdb
• Descriptor: NITRILE HYDRATASE (SUBUNIT ALPHA), NITRILE HYDRATASE (SUBUNIT BETA), FE (III) ION (3 entities in total)
• Functional Keywords: nitrile hydratase, iron center, non-heme iron, lyase
• Biological source: Rhodococcus sp. R312; More
• Total number of polymer chains: 8
• Total formula weight: 186248.66

Authors

Huang, W.,Schneider, G.,Lindqvist, Y. (deposition date: 1997-04-05, release date: 1998-04-08, Last modification date: 2024-10-30)

Primary citation

Huang, W.,Jia, J.,Cummings, J.,Nelson, M.,Schneider, G.,Lindqvist, Y.
Crystal structure of nitrile hydratase reveals a novel iron centre in a novel fold.
Structure, 5:691-699, 1997

PubMed Abstract: Nitrile hydratases are unusual metalloenzymes that catalyze the hydration of nitriles to their corresponding amides. They are used as biocatalysts in acrylamide production, one of the few commercial scale bioprocesses, as well as in environmental remediation for the removal of nitriles from waste streams. Nitrile hydratases are composed of two subunits, alpha and beta, and they contain one iron atom per alphabeta unit. We have determined the crystal structure of photoactivated iron-containing nitrile hydratase from Rhodococcus sp. R312 to 2.65 A resolution as a first step in the elucidation of its catalytic mechanism.

PubMed: 9195885
DOI: 10.1016/S0969-2126(97)00223-2

Experimental method

X-RAY DIFFRACTION (2.65 Å)