1A5B

CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHA D60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49

1A5B の概要

• エントリーDOI: 10.2210/pdb1a5b/pdb
• 分子名称: TRYPTOPHAN SYNTHASE (ALPHA CHAIN), TRYPTOPHAN SYNTHASE (BETA CHAIN), INDOLE-3-GLYCEROL PHOSPHATE, ... (6 entities in total)
• 機能のキーワード: carbon-oxygen lyase, mutation at position 60 (asp --> asn) in the a-subunit, true substrate indole-3-glycerol phosphate in the a-subunit, lyase
• 由来する生物種: Salmonella typhimurium; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72244.30

構造登録者

Rhee, S.,Miles, E.W.,Davies, D.R. (登録日: 1998-02-12, 公開日: 1998-05-27, 最終更新日: 2021-11-03)

• 主引用文献: Rhee, S.,Miles, E.W.,Davies, D.R.; Cryo-crystallography of a true substrate, indole-3-glycerol phosphate, bound to a mutant (alphaD60N) tryptophan synthase alpha2beta2 complex reveals the correct orientation of active site alphaGlu49.; J.Biol.Chem., 273:8553-8555, 1998; PubMed: 9535826; DOI: 10.1074/jbc.273.15.8553

実験手法

X-RAY DIFFRACTION (2 Å)