1A54

PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN FLUOROPHORE AND BOUND TO DIHYDROGENPHOSPHATE ION

Summary for 1A54

DescriptorPHOSPHATE-BINDING PROTEIN, DIHYDROGENPHOSPHATE ION, N-[2-(1-MALEIMIDYL)ETHYL]-7-DIETHYLAMINOCOUMARIN-3-CARBOXAMIDE, ... (4 entities in total)
Functional Keywordsphosphotransferase, transport, coumarin, fluorophore
Biological sourceEscherichia coli
Total number of polymer chains1
Total molecular weight35353.45
Authors
Hirshberg, M.,Henrick, K.,Lloyd-Haire, L.,Vasisht, N.,Brune, M.,Corrie, J.E.T.,Webb, M.R. (deposition date: 1998-02-19, release date: 1998-10-14, Last modification date: 2011-07-13)
Primary citation
Hirshberg, M.,Henrick, K.,Haire, L.L.,Vasisht, N.,Brune, M.,Corrie, J.E.,Webb, M.R.
Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate.
Biochemistry, 37:10381-10385, 1998
PubMed: 9671506 (PDB entries with the same primary citation)
DOI: 10.1021/bi980428z
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.6 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliers402.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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