1A54
PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN FLUOROPHORE AND BOUND TO DIHYDROGENPHOSPHATE ION
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 102 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.498, 62.651, 122.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.600 |
R-factor | 0.177 * |
Rwork | 0.177 |
R-free | 0.20800 |
Structure solution method | MR |
Starting model (for MR) | 2abh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.859 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CCP4 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.080 | 0.210 |
Total number of observations | 345731 * | |
Number of reflections | 40945 * | |
Completeness [%] | 95.5 | 93.4 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 * | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | reservoir | PEG6000 | 20-24 (%(w/v)) | |
4 | 1 | reservoir | potassium acetate | 20 (mM) | |
5 | 1 | reservoir | potassium phosphate | 2 (mM) | |
6 | 1 | reservoir | 50 (mM) |