1A0H

THE X-RAY CRYSTAL STRUCTURE OF PPACK-MEIZOTHROMBIN DESF1: KRINGLE/THROMBIN AND CARBOHYDRATE/KRINGLE/THROMBIN INTERACTIONS AND LOCATION OF THE LINKER CHAIN

Summary for 1A0H

• Entry DOI: 10.2210/pdb1a0h/pdb
• Related PRD ID: PRD_000020
• Descriptor: MEIZOTHROMBIN, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide, ... (5 entities in total)
• Functional Keywords: serine protease, coagulation, thrombin, prothrombin, meizothrombin, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• Biological source: Bos taurus (cattle); More
• Total number of polymer chains: 4
• Total formula weight: 97038.29

Authors

Martin, P.D.,Malkowski, M.G.,Box, J.,Esmon, C.T.,Edwards, B.F.P. (deposition date: 1997-11-30, release date: 1998-06-17, Last modification date: 2024-11-13)

Primary citation

Martin, P.D.,Malkowski, M.G.,Box, J.,Esmon, C.T.,Edwards, B.F.
New insights into the regulation of the blood clotting cascade derived from the X-ray crystal structure of bovine meizothrombin des F1 in complex with PPACK.
Structure, 5:1681-1693, 1997

PubMed Abstract: The conversion of prothrombin to thrombin by factor Xa is the penultimate step in the blood clotting cascade. In vivo, where the conversion occurs primarily on activated platelets in association with factor Va and Ca2+ ions, meizothrombin is the major intermediate of the two step reaction. Meizothrombin rapidly loses the fragment 1 domain (F1) by autolysis to become meizothrombin des F1 (mzTBN-F1). The physiological properties of mzTBN-F1 differ dramatically from those of thrombin due to the presence of prothrombin fragment 2 (F2), which remains covalently attached to the activated thrombin domain in mzTBN-F1.

PubMed: 9438869
DOI: 10.1016/S0969-2126(97)00314-6

Experimental method

X-RAY DIFFRACTION (3.2 Å)