1A02

STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA

1A02 の概要

• エントリーDOI: 10.2210/pdb1a02/pdb
• 分子名称: DNA (5'-D(*DTP*DTP*DGP*DGP*DAP*DAP*DAP*DAP*DTP*DTP*DTP*DGP*DTP*DTP*DTP*DCP*DAP*DTP*DAP*DG)-3'), DNA (5'-D(*DAP*DAP*DCP*DTP*DAP*DTP*DGP*DAP*DAP*DAP*DCP*DAP*DAP*DAP*DTP*DTP*DTP*DTP*DCP*DC)-3'), NUCLEAR FACTOR OF ACTIVATED T CELLS, ... (6 entities in total)
• 機能のキーワード: transcription factor, nfat, nf-at, ap-1, fos-jun, quaternary protein-dna complex, transcription synergy, combinatorial gene regulation, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q13469; Nucleus: P01100 P05412
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 59845.47

構造登録者

Chen, L.,Glover, J.N.M.,Hogan, P.G.,Rao, A.,Harrison, S.C. (登録日: 1997-12-08, 公開日: 1998-05-27, 最終更新日: 2024-02-07)

主引用文献

Chen, L.,Glover, J.N.,Hogan, P.G.,Rao, A.,Harrison, S.C.
Structure of the DNA-binding domains from NFAT, Fos and Jun bound specifically to DNA.
Nature, 392:42-48, 1998

PubMed Abstract: The nuclear factor of activated T cells (NFAT) and the AP-1 heterodimer, Fos-Jun, cooperatively bind a composite DNA site and synergistically activate the expression of many immune-response genes. A 2.7-A-resolution crystal structure of the DNA-binding domains of NFAT, Fos and Jun, in a quaternary complex with a DNA fragment containing the distal antigen-receptor response element from the interleukin-2 gene promoter, shows an extended interface between NFAT and AP-1, facilitated by the bending of Fos and DNA. The tight association of the three proteins on DNA creates a continuous groove for the recognition of 15 base pairs.

PubMed: 9510247
DOI: 10.1038/32100

実験手法

X-RAY DIFFRACTION (2.7 Å)