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1TWX

Crystal structure of the thrombin mutant D221A/D222K

Summary for 1TWX
Entry DOI10.2210/pdb1twx/pdb
DescriptorProthrombin, Hirudin, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
Functional Keywordsthrombin, hydrolase, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
Biological sourceHomo sapiens (human)
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Total number of polymer chains3
Total formula weight34566.56
Authors
Pineda, A.O.,Zhang, E.,Guinto, E.R.,Savvides, S.N.,Tulinsky, A.,Di Cera, E. (deposition date: 2004-07-01, release date: 2005-04-19, Last modification date: 2023-11-15)
Primary citationPineda, A.O.,Zhang, E.,Guinto, E.R.,Savvides, S.N.,Tulinsky, A.,Di Cera, E.
Crystal structure of the thrombin mutant D221A/D222K: the Asp222:Arg187 ion-pair stabilizes the fast form
Biophys.Chem., 112:253-256, 2004
Cited by
PubMed Abstract: The thrombin mutant D221A/D222K (ARK) does not bind Na+ and has interesting functional properties intermediate between those of the slow and fast forms of wild type. We solved the X-ray crystal structure of ARK bound at exosite I with a fragment of hirudin at 2.4-A resolution. The structure shows a slight collapse of the 186 and 220 loops into the Na+ binding site due to disruption of the Asp222:Arg187 ion-pair. The backbone O atoms of Arg221a and Lys224 are shifted into conformations that eliminate optimal interaction with Na+. A paucity of solvent molecules in the Na+ binding site is also noted, by analogy to what is seen in the structure of the slow form. These findings reinforce the crucial role of the Asp222:Arg187 ion-pair in stabilizing the fast form of thrombin.
PubMed: 15572256
DOI: 10.1016/j.bpc.2004.07.027
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.4 Å)
Structure validation

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