1Q2B

CELLOBIOHYDROLASE CEL7A WITH DISULPHIDE BRIDGE ADDED ACROSS EXO-LOOP BY MUTATIONS D241C AND D249C

Summary for 1Q2B

• Entry DOI: 10.2210/pdb1q2b/pdb
• Related: 1EGN 1Q2E 2CEL 3CEL 5CEL 6CEL 7CEL
• Descriptor: EXOCELLOBIOHYDROLASE I, 2-acetamido-2-deoxy-beta-D-glucopyranose, COBALT (II) ION, ... (4 entities in total)
• Functional Keywords: hydrolase, cellulase, cellulose degradation, glycosidase, glycoprotein, glycosylated protein, disulphide mutant
• Biological source: Hypocrea jecorina
• Total number of polymer chains: 1
• Total formula weight: 46383.92

Authors

Stahlberg, J.,Harris, M.,Jones, T.A. (deposition date: 2003-07-24, release date: 2003-11-25, Last modification date: 2024-11-20)

Primary citation

von Ossowski, I.,Stahlberg, J.,Koivula, A.,Piens, K.,Becker, D.,Boer, H.,Harle, R.,Harris, M.,Divne, C.,Mahdi, S.,Zhao, Y.,Driguez, H.,Claeyssens, M.,Sinnott, M.L.,Teeri, T.T.
Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D.
J.Mol.Biol., 333:817-829, 2003

PubMed Abstract: The exo-loop of Trichoderma reesei cellobiohydrolase Cel7A forms the roof of the active site tunnel at the catalytic centre. Mutants were designed to study the role of this loop in crystalline cellulose degradation. A hydrogen bond to substrate made by a tyrosine at the tip of the loop was removed by the Y247F mutation. The mobility of the loop was reduced by introducing a new disulphide bridge in the mutant D241C/D249C. The tip of the loop was deleted in mutant Delta(G245-Y252). No major structural disturbances were observed in the mutant enzymes, nor was the thermostability of the enzyme affected by the mutations. The Y247F mutation caused a slight k(cat) reduction on 4-nitrophenyl lactoside, but only a small effect on cellulose hydrolysis. Deletion of the tip of the loop increased both k(cat) and K(M) and gave reduced product inhibition. Increased activity was observed on amorphous cellulose, while only half the original activity remained on crystalline cellulose. Stabilisation of the exo-loop by the disulphide bridge enhanced the activity on both amorphous and crystalline cellulose. The ratio Glc(2)/(Glc(3)+Glc(1)) released from cellulose, which is indicative of processive action, was highest with Tr Cel7A wild-type enzyme and smallest with the deletion mutant on both substrates. Based on these data it seems that the exo-loop of Tr Cel7A has evolved to facilitate processive crystalline cellulose degradation, which does not require significant conformational changes of this loop.

PubMed: 14568538
DOI: 10.1016/S0022-2836(03)00881-7

Experimental method

X-RAY DIFFRACTION (1.6 Å)