1Q2B
CELLOBIOHYDROLASE CEL7A WITH DISULPHIDE BRIDGE ADDED ACROSS EXO-LOOP BY MUTATIONS D241C AND D249C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.986 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.170, 83.140, 110.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| Rwork | 0.207 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 25.200 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.147 |
| Number of reflections | 46521 | |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 92.0 | 95.8 |
| Redundancy | 4.0 * | 3.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 298 | PEG 5000 monomethyl ether, sodium morpholine-ethane-sulphonic acid, glycerol, cobalt chloride, sodium acetate, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1-5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 10 (mM) | pH5.0 |
| 3 | 1 | reservoir | mPEG5000 | 18-22 (%(w/v)) | |
| 4 | 1 | reservoir | sodium morpholine ethane sulphonic acid | 0.1 (M) | pH6.0 |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | glycerol | 12.5 (%(v/v)) |
