1OYY

Structure of the RecQ Catalytic Core bound to ATP-gamma-S

Summary for 1OYY

• Entry DOI: 10.2210/pdb1oyy/pdb
• Related: 1OWY
• Descriptor: ATP-dependent DNA helicase, ZINC ION, MANGANESE (II) ION, ... (5 entities in total)
• Functional Keywords: recq, helicase, winged helix, helix-turn-helix, atp binding, zn(2+) binding, atp(gamma)s, hydrolase
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 59428.72

Authors

Bernstein, D.A.,Zittel, M.C.,Keck, J.L. (deposition date: 2003-04-07, release date: 2003-10-07, Last modification date: 2023-09-20)

Primary citation

Bernstein, D.A.,Zittel, M.C.,Keck, J.L.
High-resolution structure of the E. coli RecQ helicase catalytic core
Embo J., 22:4910-4921, 2003

PubMed Abstract: RecQ family helicases catalyze critical genome maintenance reactions in bacterial and eukaryotic cells, playing key roles in several DNA metabolic processes. Mutations in recQ genes are linked to genome instability and human disease. To define the physical basis of RecQ enzyme function, we have determined a 1.8 A resolution crystal structure of the catalytic core of Escherichia coli RecQ in its unbound form and a 2.5 A resolution structure of the core bound to the ATP analog ATPgammaS. The RecQ core comprises four conserved subdomains; two of these combine to form its helicase region, while the others form unexpected Zn(2+)-binding and winged-helix motifs. The structures reveal the molecular basis of missense mutations that cause Bloom's syndrome, a human RecQ-associated disease. Finally, based on findings from the structures, we propose a mechanism for RecQ activity that could explain its functional coordination with topoisomerase III.

PubMed: 14517231
DOI: 10.1093/emboj/cdg500

Experimental method

X-RAY DIFFRACTION (2.5 Å)