1OYY
Structure of the RecQ Catalytic Core bound to ATP-gamma-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.591, 54.534, 78.686 |
| Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.210 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 3.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 * | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.048 * | 0.133 * |
| Total number of observations | 47206 * | |
| Number of reflections | 17529 * | |
| <I/σ(I)> | 12.8 | 7.1 |
| Completeness [%] | 92.1 * | 92.1 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | PEG 1000, PEG 400, MES, Ammonium Sulfate, Manganese Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 110K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | reservoir | PEG1000 | 0.5 (%) | |
| 4 | 1 | reservoir | PEG400 | 2-4 (%) | |
| 5 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
| 6 | 1 | reservoir | ammonium sulfate | 50 (mM) |
