1MH0
Crystal structure of the anticoagulant slow form of thrombin
Summary for 1MH0
| Entry DOI | 10.2210/pdb1mh0/pdb |
| Descriptor | Prothrombin, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total) |
| Functional Keywords | thrombin, allostery, sodium binding, serine protease, blood clotting |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 2 |
| Total formula weight | 66458.17 |
| Authors | Pineda, A.O.,Savvides, S.,Waksman, G.,Di Cera, E. (deposition date: 2002-08-18, release date: 2002-11-08, Last modification date: 2024-11-13) |
| Primary citation | Pineda, A.O.,Savvides, S.,Waksman, G.,Di Cera, E. Crystal structure of the anticoagulant slow form of thrombin J.Biol.Chem., 277:40177-40180, 2002 Cited by PubMed Abstract: Using the thrombin mutant R77aA devoid of the site of autoproteolytic degradation at exosite I, we have solved for the first time the structure of thrombin free of any inhibitors and effector molecules and stabilized in the Na(+)-free slow form. The slow form shows subtle differences compared with the currently available structures of the Na(+)-bound fast form that carry inhibitors at the active site or exosite I. The most notable differences are the displacement of Asp-189 in the S1 specificity pocket, a downward shift of the 190-193 strand, a rearrangement of the side chain of Glu-192, and a significant shift in the position of the catalytic Ser-195 that is no longer within H-bonding distance from His-57. The structure of the slow form explains the reduced specificity toward synthetic and natural substrates and suggests a molecular basis for its anticoagulant properties. PubMed: 12205081DOI: 10.1074/jbc.C200465200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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