1M1F

Kid toxin protein from E.coli plasmid R1

Summary for 1M1F

• Entry DOI: 10.2210/pdb1m1f/pdb
• Related: 4VUB
• Descriptor: Kid toxin protein, PHOSPHATE ION (3 entities in total)
• Functional Keywords: toxin-antitoxin, plasmid maintenance, post segregational killing, dna replication, mutational analysis, ccdb, toxin
• Biological source: Escherichia coli
• Total number of polymer chains: 2
• Total formula weight: 23886.50

Authors

Hargreaves, D.,Santos-Sierra, S.,Giraldo, R.,Sabariegos-Jareno, R.,de la Cueva-Mendez, G.,Boelens, R.,Diaz-Orejas, R.,Rafferty, J.B. (deposition date: 2002-06-19, release date: 2002-11-20, Last modification date: 2024-02-14)

Primary citation

Hargreaves, D.,Santos-Sierra, S.,Giraldo, R.,Sabariegos-Jareno, R.,de la Cueva-Mendez, G.,Boelens, R.,Diaz-Orejas, R.,Rafferty, J.B.
Structural and Functional Analysis of the Kid toxin protein from E.coli plasmid R1
Structure, 10:1425-1433, 2002

PubMed Abstract: We have determined the structure of Kid toxin protein from E. coli plasmid R1 involved in stable plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Kid forms a two-component system with its antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid despite the lack of any notable sequence similarity. Analysis of nontoxic mutants of Kid suggests a target interaction interface associated with toxicity that is in marked contrast to that proposed for CcdB. A possible region for interaction of Kid with the antitoxin is proposed that overlaps with the target binding site and may explain the mode of antitoxin action.

PubMed: 12377128
DOI: 10.1016/S0969-2126(02)00856-0

Experimental method

X-RAY DIFFRACTION (1.4 Å)