1JR3

Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III

Summary for 1JR3

• Entry DOI: 10.2210/pdb1jr3/pdb
• Related: 1A5T 1JQJ 1JQL 2POL
• Descriptor: DNA polymerase III subunit gamma, DNA polymerase III, delta subunit, DNA polymerase III, delta' subunit, ... (5 entities in total)
• Functional Keywords: dna polymerase, processivity, processivity clamp, clamp loader, aaa+ atpase, transferase
• Biological source: Escherichia coli; More
• Total number of polymer chains: 5
• Total formula weight: 200808.45

Authors

Jeruzalmi, D.,O'Donnell, M.,Kuriyan, J. (deposition date: 2001-08-10, release date: 2001-09-26, Last modification date: 2024-02-07)

Primary citation

Jeruzalmi, D.,O'Donnell, M.,Kuriyan, J.
Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III.
Cell(Cambridge,Mass.), 106:429-441, 2001

PubMed Abstract: The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC) that loads the sliding clamp (beta, homologous to PCNA) onto DNA. The 2.7/3.0 A crystal structure of gamma complex reveals a pentameric arrangement of subunits, with stoichiometry delta':gamma(3):delta. The C-terminal domains of the subunits form a circular collar that supports an asymmetric arrangement of the N-terminal ATP binding domains of the gamma motor and the structurally related domains of the delta' stator and the delta wrench. The structure suggests a mechanism by which the gamma complex switches between a closed state, in which the beta-interacting element of delta is hidden by delta', and an open form similar to the crystal structure, in which delta is free to bind to beta.

PubMed: 11525729
DOI: 10.1016/S0092-8674(01)00463-9

Experimental method

X-RAY DIFFRACTION (2.7 Å)