1JR3

Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	80
Detector technology	CCD
Collection date	2000-10-17
Detector	CUSTOM-MADE
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	95.698, 95.857, 285.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.700
R-factor	0.268 *
Rwork	0.268
R-free	0.30430
Structure solution method	MIR
RMSD bond length	0.001 *
RMSD bond angle	1.720 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	1000.000
High resolution limit [Å]	2.700	2.700
Rmerge	0.136 *
Total number of observations	552088 *
Number of reflections	60657
Completeness [%]	83.0	93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	294	Polyethylene glycol, ammonium sulphate, dithiothreitol, dimethylacetamide, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 21K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	20 (mM)
2	1	1	dithiothreitol	2 (mM)
3	1	1	protein	100 (mg/ml)
4	1	2	HEPES	50 (mM)
5	1	2	ammonium sulfate	1.05-1.15 (M)
6	1	2	PEG400	6-7.5 (%)
7	1	2	N,N-dimethylacetamide	320 (mM)
8	1	2	dithiothreitol	4 (mM)