1A76

FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII

Summary for 1A76

• Entry DOI: 10.2210/pdb1a76/pdb
• Descriptor: FLAP ENDONUCLEASE-1 PROTEIN, MANGANESE (II) ION (3 entities in total)
• Functional Keywords: 5'-3' exo/endo nuclease, dna replication, rth, rad27, dna repair, 5'-3' exo-endo nuclease complex
• Biological source: Methanocaldococcus jannaschii
• Total number of polymer chains: 1
• Total formula weight: 37695.35

Authors

Hwang, K.Y.,Baek, K.,Kim, H.,Cho, Y. (deposition date: 1998-03-20, release date: 1999-08-03, Last modification date: 2024-02-07)

Primary citation

Hwang, K.Y.,Baek, K.,Kim, H.Y.,Cho, Y.
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii.
Nat.Struct.Biol., 5:707-713, 1998

PubMed Abstract: Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.

PubMed: 9699635
DOI: 10.1038/1406

Experimental method

X-RAY DIFFRACTION (2 Å)