1A76

FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	58.780, 42.800, 62.640
Unit cell angles	90.00, 92.26, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.214
Rwork	0.214
R-free	0.27900
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	25.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.056	0.144
Total number of observations	76416 *
Number of reflections	20682
<I/σ(I)>	19.3	5
Completeness [%]	92.4	88.7
Redundancy	4.3	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	7 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%)
2	1	reservoir		5 (mM)
3	1	reservoir	HEPES/Na	100 (mM)