12AG

Spermine-blocked open-state cryo-EM structure of human TRPV6 channel in cNW30 nanodiscs

Summary for 12AG

• Entry DOI: 10.2210/pdb12ag/pdb
• EMDB information: 76261
• Descriptor: Transient receptor potential cation channel subfamily V member 6, CHOLESTEROL HEMISUCCINATE, 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (6 entities in total)
• Functional Keywords: polyamine, spermine, cancer, cryo-em, trp channels, trpv6, oncochannel, channel block, membrane protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 4
• Total formula weight: 298390.73

Authors

Neuberger, A.,Sobolevsky, A.I. (deposition date: 2026-03-23, release date: 2026-06-10)

Primary citation

Neuberger, A.,Veretenenko, I.I.,Shalygin, A.,Trofimov, Y.A.,Gudermann, T.,Chubanov, V.,Efremov, R.G.,Sobolevsky, A.I.
Open-channel block of human TRPV6 by polyamine spermine.
Nat Commun, 17:-, 2026

PubMed Abstract: Polyamines are organic cations that are present at sub-millimolar concentrations in the cytoplasm and extracellular fluids and serve as versatile modulators of TRP channels, fine-tuning their functions in physiological and pathological contexts, including pain, inflammation and cancer. Despite extensive functional studies, the structural basis by which polyamines regulate TRP channels remains unclear. Here, we combine calcium imaging, electrophysiology, cryo-electron microscopy, mutagenesis and molecular dynamics simulations to study regulation of human TRPV6 by polyamine spermine. Our functional experiments demonstrate voltage-dependent block of TRPV6-mediated currents by spermine. Cryo-electron microscopy reveals that spermine binds in the open pore of TRPV6, extending along the pore axis through the selectivity filter and central cavity. Mutagenesis and molecular dynamics simulations confirm the main binding site of spermine in the selectivity filter and suggest a stepwise molecular mechanism of channel block that includes two more binding sites in the pore transiently occupied by spermine. Our findings enrich the knowledge about TRPV6 regulation by endogenous factors and provide details of the ion channel blocking mechanism that can be explored for inhibition of this channel in disease conditions.

PubMed: 42204171
DOI: 10.1038/s41467-026-73653-5

Experimental method

ELECTRON MICROSCOPY (3.48 Å)