9ZP4
E. coli RNA polymerase elongation complex containing the unnatural dZ:PTP base pair in a trigger-loop-closed conformation
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, G) | DNA-directed RNA polymerase subunit alpha | polymer | 231 | 25526.0 | 2 | UniProt (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1430 | 158105.7 | 1 | UniProt (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (K) | DNA-directed RNA polymerase subunit omega | polymer | 74 | 8452.6 | 1 | UniProt (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (N) | Non-template strand DNA | polymer | 11 | 3407.2 | 1 | Escherichia coli | ||
| 6 | G (R) | RNA | polymer | 9 | 2934.8 | 1 | Escherichia coli | ||
| 7 | H (T) | Template strand DNA | polymer | 22 | 6674.3 | 1 | Escherichia coli | ||
| 8 | I, J (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 9 | K, L (J) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 10 | M (J) | (5S)-2-amino-8-{5-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}imidazo[1,2-a][1,3,5]triazin-4(8H)-one | non-polymer | 523.2 | 1 | Chemie (A1C5A) | |||
| 11 | N, O, P, Q, R... (A, G, I, J, K...) | water | water | 18.0 | 641 | Chemie (HOH) |
Sequence modifications
J: 1 - 1407 (UniProt: P0A8T7)
| PDB | External Database | Details |
|---|---|---|
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Leu 1410 | - | expression tag |
| Glu 1411 | - | expression tag |
| Val 1412 | - | expression tag |
| Leu 1413 | - | expression tag |
| Phe 1414 | - | expression tag |
| Gln 1415 | - | expression tag |
| Gly 1416 | - | expression tag |
| Pro 1417 | - | expression tag |
| Ser 1418 | - | expression tag |
| Ser 1419 | - | expression tag |
| Gly 1420 | - | expression tag |
| His 1421 | - | expression tag |
| His 1422 | - | expression tag |
| His 1423 | - | expression tag |
| His 1424 | - | expression tag |
| His 1425 | - | expression tag |
| His 1426 | - | expression tag |
| His 1427 | - | expression tag |
| His 1428 | - | expression tag |
| His 1429 | - | expression tag |
| His 1430 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 381447.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 702.6 | |
| All* | Total formula weight | 382150.2 |
