9ZOH
Cryo-EM structure of the complete Sulfolobus acidocaldarius RNA polymerase in open clamp conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit Rpo1N | polymer | 880 | 99929.1 | 1 | UniProt (P11512) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit A,DNA-directed RNA polymerase subunit A',Rpo1' |
| 2 | B (B) | DNA-directed RNA polymerase subunit Rpo2 | polymer | 1126 | 126647.1 | 1 | UniProt (P11513) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit B |
| 3 | C (C) | DNA-directed RNA polymerase subunit Rpo1C | polymer | 393 | 44507.4 | 1 | UniProt (P11514) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit A'',DNA-directed RNA polymerase subunit C,Rpo1'' |
| 4 | D (E) | DNA-directed RNA polymerase subunit Rpo7 | polymer | 183 | 20496.8 | 1 | UniProt (P39466) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit E |
| 5 | E (G) | DNA-directed RNA polymerase subunit Rpo8 | polymer | 124 | 14463.9 | 1 | UniProt (Q4JAY4) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase,subunit G |
| 6 | F (H) | DNA-directed RNA polymerase subunit Rpo5 | polymer | 84 | 9477.1 | 1 | UniProt (P11521) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit H |
| 7 | G (K) | DNA-directed RNA polymerase subunit Rpo6 | polymer | 89 | 10252.0 | 1 | UniProt (P39463) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit K |
| 8 | H (L) | DNA-directed RNA polymerase subunit Rpo11 | polymer | 90 | 10061.8 | 1 | UniProt (P46217) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit L |
| 9 | I (N) | DNA-directed RNA polymerase subunit Rpo10 | polymer | 66 | 7673.1 | 1 | UniProt (P39472) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit N |
| 10 | J (Y) | DNA-directed RNA polymerase subunit Rpo13 | polymer | 105 | 12331.7 | 1 | UniProt (Q4JAJ6) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit F |
| 11 | K (D) | DNA-directed RNA polymerase subunit Rpo3 | polymer | 264 | 29858.8 | 1 | UniProt (P39471) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit D |
| 12 | L (P) | DNA-directed RNA polymerase subunit Rpo12 | polymer | 48 | 5662.9 | 1 | UniProt (Q4JAE8) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit P |
| 13 | M (F) | DNA-directed RNA polymerase subunit Rpo4 | polymer | 114 | 13071.0 | 1 | UniProt (Q4JB12) | Sulfolobus acidocaldarius DSM 639 | DNA-directed RNA polymerase subunit F |
| 14 | N, O, P, Q, S (A, B, N, P) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) PubChem (32051) | |||
| 15 | R (D) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) |
Sequence modifications
B: 1 - 1126 (UniProt: P11513)
G: 4 - 124 (UniProt: Q4JAY4)
| PDB | External Database | Details |
|---|---|---|
| Ile 772 | Val 772 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Lys 2 | - | expression tag |
| Glu 3 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 404432.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 678.7 | |
| All* | Total formula weight | 405111.2 |
