9ZO4
Cryo-EM structure of the complete Pyrococcus furiosus RNA polymerase in closed clamp conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit Rpo1N | polymer | 907 | 103265.8 | 1 | UniProt (Q8U0M4) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit A' |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1117 | 127188.9 | 1 | UniProt (Q8U0M3) | Pyrococcus furiosus DSM 3638 | |
| 3 | C (C) | DNA-directed RNA polymerase subunit Rpo1C | polymer | 397 | 44468.2 | 1 | UniProt (Q8U0M5) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit A'' |
| 4 | D (D) | DNA-directed RNA polymerase subunit Rpo3 | polymer | 275 | 31688.2 | 1 | UniProt (Q8U0E4) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit D |
| 5 | E (E) | DNA-directed RNA polymerase subunit Rpo7 | polymer | 189 | 21731.5 | 1 | UniProt (Q8U439) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit E |
| 6 | F (F) | DNA-directed RNA polymerase subunit Rpo4 | polymer | 120 | 14116.3 | 1 | UniProt (Q8U216) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit F |
| 7 | G (H) | DNA-directed RNA polymerase subunit Rpo5 | polymer | 82 | 9260.8 | 1 | UniProt (Q8U0M2) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit H |
| 8 | H (K) | DNA-directed RNA polymerase subunit Rpo6 | polymer | 57 | 6243.5 | 1 | UniProt (Q8U0E8) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit K |
| 9 | I (L) | DNA-directed RNA polymerase subunit Rpo11 | polymer | 95 | 11132.8 | 1 | UniProt (Q8U4N1) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit L |
| 10 | J (N) | DNA-directed RNA polymerase subunit Rpo10 | polymer | 65 | 7800.2 | 1 | UniProt (P60292) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit N |
| 11 | K (P) | DNA-directed RNA polymerase subunit Rpo12 | polymer | 49 | 5770.1 | 1 | UniProt (Q8TZI3) | Pyrococcus furiosus DSM 3638 | DNA-directed RNA polymerase subunit P |
| 12 | L, M, N, O, P (A, B, N, P) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) PubChem (32051) |
Sequence modifications
D: 1 - 261 (UniProt: Q8U0E4)
| PDB | External Database | Details |
|---|---|---|
| Trp 262 | - | expression tag |
| Ser 263 | - | expression tag |
| His 264 | - | expression tag |
| Pro 265 | - | expression tag |
| Gln 266 | - | expression tag |
| Phe 267 | - | expression tag |
| Glu 268 | - | expression tag |
| Lys 269 | - | expression tag |
| His 270 | - | expression tag |
| His 271 | - | expression tag |
| His 272 | - | expression tag |
| His 273 | - | expression tag |
| His 274 | - | expression tag |
| His 275 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 382666.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 327.0 | |
| All* | Total formula weight | 382993.2 |
