9ZO4
Cryo-EM structure of the complete Pyrococcus furiosus RNA polymerase in closed clamp conformation
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.05 |
| Software | cryoSPARC,PHENIX,UCSF ChimeraX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
