9Z3D

CryoEM structure of human NSUN2(C271A) with SAH cross-linked to a pre-tRNA Leu(CAA) intron

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA cytosine C(5)-methyltransferase NSUN2	polymer	782	88382.6	1	UniProt (Q08J23)	Homo sapiens (human)	Myc-induced SUN domain-containing protein,Misu,NOL1/NOP2/Sun domain family member 2,Substrate of AIM1/Aurora kinase B,mRNA cytosine C(5)-methyltransferase,tRNA cytosine C(5)-methyltransferase,tRNA methyltransferase 4 homolog,hTrm4
2	B (B)	RNA (26-MER)	polymer	31	9879.9	1		Homo sapiens
3	C (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH) PubChem (439155)

Sequence modifications

A: 2 - 767 (UniProt: Q08J23)

PDB	External Database	Details
Met -14	-	initiating methionine
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Ala 271	Cys 271	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98262.5
Non-Polymers*	Number of molecules	1
	Total formula weight	384.4
All*	Total formula weight	98646.9

*Water molecules are not included.