9Z3D

CryoEM structure of human NSUN2(C271A) with SAH cross-linked to a pre-tRNA Leu(CAA) intron

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Phase Plate	VOLTA PHASE PLATE
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.96
Number of Particle	884000
Software	cryoSPARC,SerialEM,Coot,PHENIX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION