9YRK
Cryo-EM structure of SARS-CoV-2 nsp10-nsp14 (E191A) in complex with T20P14-B complex, dimeric form
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Non-structural protein 10 | polymer | 139 | 14802.9 | 2 | UniProt (P0DTD1) | Severe acute respiratory syndrome coronavirus 2 | nsp10,Growth factor-like peptide,GFL |
| 2 | B, D (B, D) | Proofreading exoribonuclease | polymer | 527 | 59829.4 | 2 | UniProt (P0DTD1) | Severe acute respiratory syndrome coronavirus 2 | ExoN,Guanine-N7 methyltransferase,Non-structural protein 14,nsp14 |
| 3 | E (E) | T20P14-B RNA | polymer | 38 | 12187.3 | 1 | Severe acute respiratory syndrome coronavirus 2 | ||
| 4 | F (F) | P6 RNA | polymer | 6 | 1788.1 | 1 | Severe acute respiratory syndrome coronavirus 2 | ||
| 5 | G, H, J, K, L... (A, B, C, D) | ZINC ION | non-polymer | 65.4 | 10 | Chemie (ZN) | |||
| 6 | I, O (B, D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | S (E) | [(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-fluoranyl-4-methyl-3-oxidanyl-oxolan-2-yl]methyl dihydrogen phosphate | non-polymer | 379.2 | 1 | Chemie (EIF) |
Sequence modifications
B, D: 1 - 527 (UniProt: P0DTD1)
| PDB | External Database | Details |
|---|---|---|
| Ala 191 | Glu 6116 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 163240.1 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1081.9 | |
| All* | Total formula weight | 164322.0 |
