9YNQ
Cryo-EM structure of Escherichia coli transcription initiation complex with GpA and des-hydroxy pseudouridimycin (des-hydroxy PUM)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) | Escherichia coli str. K-12 substr. MG1655 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) | Escherichia coli str. K-12 substr. MG1655 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) | Escherichia coli str. K-12 substr. MG1655 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) | Escherichia coli str. K-12 substr. MG1655 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (P00579) | Escherichia coli str. K-12 substr. MG1655 | Sigma-70 |
| 6 | G (G) | DNA (27-MER) | polymer | 27 | 8421.4 | 1 | Escherichia coli str. K-12 substr. MG1655 | ||
| 7 | H (H) | DNA | polymer | 21 | 6464.2 | 1 | Escherichia coli str. K-12 substr. MG1655 | ||
| 8 | I (I) | RNA (5'-R(*GP*A)-3') | polymer | 2 | 629.5 | 1 | Escherichia coli str. K-12 substr. MG1655 | ||
| 9 | J, K (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | L (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 11 | M (I) | (1S)-1,4-anhydro-5-[(N-carbamimidoylglycyl-L-glutaminyl)amino]-5-deoxy-1-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-D-ribitol | non-polymer | 470.4 | 1 | Chemie (A1CYH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 475421.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 625.6 | |
| All* | Total formula weight | 476047.4 |
