9YNQ
Cryo-EM structure of Escherichia coli transcription initiation complex with GpA and des-hydroxy pseudouridimycin (des-hydroxy PUM)
This is a non-PDB format compatible entry.
Functional Information from PROSITE/UniProt
| site_id | PS00715 |
| Number of Residues | 14 |
| Details | SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnIGLMkAV |
| Chain | Residue | Details |
| F | ASP403-VAL416 |
| site_id | PS00716 |
| Number of Residues | 27 |
| Details | SIGMA70_2 Sigma-70 factors family signature 2. TleEVGkqfdVTrerIrQIEakaLrkL |
| Chain | Residue | Details |
| F | THR572-LEU598 |
| site_id | PS01166 |
| Number of Residues | 13 |
| Details | RNA_POL_BETA RNA polymerases beta chain signature. GdKMAGrHGNKGV |
| Chain | Residue | Details |
| C | GLY1063-VAL1075 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | Region: {"description":"Required for interaction with Crp at class II promoters"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 11 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"32871103","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4MEX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4MEY","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 19 |
| Details | DNA binding: {"description":"H-T-H motif","evidences":[{"source":"HAMAP-Rule","id":"MF_00963","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 70 |
| Details | Region: {"description":"Sigma-70 factor domain-2"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 76 |
| Details | Region: {"description":"Sigma-70 factor domain-3"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 53 |
| Details | Region: {"description":"Sigma-70 factor domain-4"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 15 |
| Details | Region: {"description":"Interaction with anti-sigma factors"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 3 |
| Details | Motif: {"description":"Interaction with polymerase core subunit RpoC"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Site: {"description":"Interaction with anti-sigma factors"} |
| Chain | Residue | Details |
