9YE9

Structure of UbcH5b in complex with the U-box domain of the E3 ubiquitin ligase CHIP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17182.7	1	UniProt (P62837) Pfam (PF00179)	Homo sapiens (human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B, C (B, C)	E3 ubiquitin-protein ligase CHIP	polymer	86	9894.2	2	UniProt (F6NGW2) Pfam (PF04564)	Danio rerio (zebrafish)	RING-type E3 ubiquitin transferase CHIP,STIP1 homology and U box-containing protein 1
3	D, E, F, H, J (A, B, C)	BETA-MERCAPTOETHANOL	non-polymer		78.1	5	Chemie (BME)
4	G, I (B, C)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	K, L, M (A, B, C)	water	water		18.0	143	Chemie (HOH)

Sequence modifications

A: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Lys 1	Met 1	engineered mutation
Lys 85	Cys 85	engineered mutation

B, C: 204 - 284 (UniProt: F6NGW2)

PDB	External Database	Details
Gly 199	-	expression tag
Ala 200	-	expression tag
Met 201	-	expression tag
Gly 202	-	expression tag
Ser 203	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	36971.2
Non-Polymers*	Number of molecules	7
	Total formula weight	461.6
All*	Total formula weight	37432.8

*Water molecules are not included.