9XZM
Cryo-EM structure of F-box helicase 1 (FBH1) bound to an SCF ubiquitin ligase complex and a 3-way DNA fork (substrate structure)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (X) | DNA (45-MER) | polymer | 45 | 13921.9 | 1 | synthetic construct | ||
| 2 | B (B) | DNA (5'-D(*TP*CP*GP*CP*TP*AP*CP*CP*TP*TP*CP*GP*CP*AP*GP*TP*C)-3') | polymer | 17 | 5114.3 | 1 | synthetic construct | ||
| 3 | C (Y) | DNA (45-MER) | polymer | 45 | 13872.9 | 1 | synthetic construct | ||
| 4 | D (A) | DNA (5'-D(*CP*TP*GP*AP*CP*GP*CP*TP*TP*CP*CP*AP*TP*CP*GP*CP*TP*GP*TP*CP*TP*AP*G)-3') | polymer | 23 | 6983.5 | 1 | synthetic construct | ||
| 5 | E (F) | F-box DNA helicase 1 | polymer | 972 | 109518.7 | 1 | UniProt (Q8NFZ0) | Homo sapiens (human) | hFBH1,DNA 3'-5' helicase 1,F-box only protein 18 |
| 6 | F (F) | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 1 | Chemie (AGS) | |||
| 7 | G (F) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 8 | H (F) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
F: 1 - 969 (UniProt: Q8NFZ0)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 149411.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 613.0 | |
| All* | Total formula weight | 150024.3 |
