9XXT
Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Probable G-protein coupled receptor 174 | polymer | 333 | 38539.5 | 1 | UniProt (Q9BXC1) Pfam (PF00001) | Homo sapiens (human) | |
| 2 | B (A) | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 372 | 43655.2 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 342 | 37516.9 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Gbeita1,Transducin beta chain 1 |
| 4 | D (N) | Nanobody35 | polymer | 128 | 13885.4 | 1 | synthetic construct | ||
| 5 | E (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | F (R) | (2~{S})-2-azanyl-3-[[(2~{R})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid | non-polymer | 525.6 | 1 | Chemie (A1E1Q) | |||
| 7 | G (R) | water | water | 18.0 | 14 | Chemie (HOH) |
Sequence modifications
A: 9 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Met 25 | Lys 25 | conflict |
| Asp 49 | Gly 49 | conflict |
| Asn 50 | Glu 50 | conflict |
| Ala 226 | Gly 212 | conflict |
| Asp 249 | Ala 235 | conflict |
| Asp 252 | Ser 238 | conflict |
| Asp 272 | Leu 258 | conflict |
| Ser 366 | Ala 352 | conflict |
| Ala 372 | Ile 358 | conflict |
| Ile 375 | Val 361 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 141458.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 525.6 | |
| All* | Total formula weight | 141983.8 |
