9X20

Cryo-EM structure of the GLP-1-bound human GLP-1R-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (P)	Glucagon-like peptide 1(7-36)	polymer	30	3302.6	1	UniProt (P01275) Pfam (PF00123)	Homo sapiens	GLP-1(7-36)
2	B (R)	Glucagon-like peptide 1 receptor	polymer	440	50860.8	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	GLP-1 receptor,GLP-1-R,GLP-1R
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	353	40313.9	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39020.7	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	67	7432.6	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (E)	scFv16	polymer	247	26293.3	1		synthetic construct

Sequence modifications

A: 2 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	167223.8
All*	Total formula weight	167223.8

*Water molecules are not included.