9WXH
Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a cognate target RNA in the pre-cleavage state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR RNA | polymer | 43 | 13718.1 | 1 | Gammaproteobacteria bacterium | ||
| 2 | B (B) | Cas10 | polymer | 592 | 67985.8 | 1 | UniProt (A0A9D0P2E0) | Gammaproteobacteria bacterium | |
| 3 | C (C) | CRISPR type III-associated protein domain-containing protein | polymer | 635 | 70691.0 | 1 | UniProt (A0A9D0P213) | Gammaproteobacteria bacterium | |
| 4 | D (D) | TIGR03986 family CRISPR-associated RAMP protein | polymer | 1235 | 138380.1 | 1 | UniProt (A0A9D0P2B2) | Gammaproteobacteria bacterium | |
| 5 | E (E) | Cognate target RNA (CTR) | polymer | 47 | 15180.1 | 1 | Gammaproteobacteria bacterium | ||
| 6 | F, G, H, K (B, C) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 7 | I (B) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 8 | J (B) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) | |||
| 9 | L (D) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) PubChem (32051) | |||
| 10 | M, N, O, P (A, C, D, E) | water | water | 18.0 | 17 | Chemie (HOH) |
Sequence modifications
B: 1 - 572 (UniProt: A0A9D0P2E0)
D: 1 - 1234 (UniProt: A0A9D0P2B2)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 305955.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1067.3 | |
| All* | Total formula weight | 307022.3 |
