9WNE
Cryo-EM structure of the LbCas12a-Lid2-crRNA-DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cpf1 | polymer | 1237 | 144912.2 | 1 | UniProt (A0A182DWE3) | Lachnospiraceae bacterium ND2006 | |
| 2 | B (B) | crRNA | polymer | 42 | 13434.0 | 1 | Lachnospiraceae bacterium ND2006 | ||
| 3 | C (C) | DNA (39-MER) | polymer | 39 | 12137.8 | 1 | Lachnospiraceae bacterium ND2006 | ||
| 4 | D (D) | DNA (39-MER) | polymer | 39 | 11870.6 | 1 | Lachnospiraceae bacterium ND2006 | ||
| 5 | E, F, G, H (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 6 | I, J (A, B) | water | water | 18.0 | 19 | Chemie (HOH) |
Sequence modifications
A: 1 - 1219 (UniProt: A0A182DWE3)
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Ser -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Asn 112 | Ala 113 | conflict |
| Glu 113 | Ala 114 | conflict |
| Phe 131 | Ala 132 | conflict |
| Leu 132 | Ala 133 | conflict |
| Gln 264 | Ala 265 | conflict |
| Lys 269 | Ala 270 | conflict |
| Val 357 | Leu 358 | conflict |
| - | Gly 931 | deletion |
| - | Phe 932 | deletion |
| - | Lys 933 | deletion |
| - | Asn 934 | deletion |
| - | Ser 935 | deletion |
| - | Arg 936 | deletion |
| - | Val 937 | deletion |
| - | Lys 938 | deletion |
| Tyr 930 | Val 939 | conflict |
| Cys 931 | Glu 940 | conflict |
| Ser 932 | Lys 941 | conflict |
| Ser 933 | Gln 942 | conflict |
| Arg 1068 | Ala 1077 | conflict |
| Asn 1069 | Ala 1078 | conflict |
| Pro 1070 | Ala 1079 | conflict |
| Asp 1077 | Ala 1086 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 182354.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 97.2 | |
| All* | Total formula weight | 182451.9 |
