9W6W

5-CT-bound serotonin 5A (5-HT5A) receptor-Gi protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	359	39475.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (E)	scFv16	polymer	258	27669.8	1		Mus musculus
5	E (R)	Soluble cytochrome b562,5-hydroxytryptamine receptor 5A	polymer	481	54103.4	1	UniProt (P0ABE7) UniProt (P47898) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,5-HT-5,5-HT-5A,5-HT5A,Serotonin receptor 5A
6	F (R)	3-(2-azanylethyl)-1H-indole-5-carboxamide	non-polymer		203.2	1	Chemie (8K3)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

R: -97 - 8 (UniProt: P0ABE7)

PDB	External Database	Details
Met -123	-	initiating methionine
Lys -122	-	expression tag
Thr -121	-	expression tag
Ile -120	-	expression tag
Ile -119	-	expression tag
Ala -118	-	expression tag
Leu -117	-	expression tag
Ser -116	-	expression tag
Tyr -115	-	expression tag
Ile -114	-	expression tag
Phe -113	-	expression tag
Cys -112	-	expression tag
Leu -111	-	expression tag
Val -110	-	expression tag
Phe -109	-	expression tag
Ala -108	-	expression tag
Asp -107	-	expression tag
Tyr -106	-	expression tag
Lys -105	-	expression tag
Asp -104	-	expression tag
Asp -103	-	expression tag
Asp -102	-	expression tag
Asp -101	-	expression tag
Ala -100	-	expression tag
Gly -99	-	expression tag
Arg -98	-	expression tag
Trp -90	Met 29	conflict
Ile 5	His 124	conflict

R: 9 - 357 (UniProt: P47898)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	169523.6
Non-Polymers*	Number of molecules	1
	Total formula weight	203.2
All*	Total formula weight	169726.8

*Water molecules are not included.