9W2K
Structural basis of substrate promiscuity in the archaeal RNA-splicing endonuclease from Candidatus Micrarchaeum acidiphilum (ARMAN-2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | tRNA intron endonuclease | polymer | 395 | 45882.7 | 2 | UniProt (C7DIA5) Pfam (PF01974) | Candidatus Micrarchaeum acidiphilum ARMAN-2 | |
| 2 | C (I) | DNA/RNA (5'-R(P*AP*CP*CP*GP*AP*CP*CP*A)-D(P*U)-R(P*AP*GP*CP*U)-3') | polymer | 13 | 4084.5 | 1 | Archaeoglobus fulgidus | ||
| 3 | D (F) | RNA (5'-R(P*AP*GP*CP*GP*GP*UP*CP*A)-3') | polymer | 8 | 2565.6 | 1 | Archaeoglobus fulgidus | ||
| 4 | E (H) | RNA (5'-R(*AP*GP*GP*U)-3') | polymer | 4 | 1280.8 | 1 | Archaeoglobus fulgidus | ||
| 5 | F, G, H, I (A, B) | ACETIC ACID | non-polymer | 60.1 | 4 | Chemie (ACY) | |||
| 6 | J (F) | ADENOSINE-5'-PHOSPHATE-2',3'-CYCLIC PHOSPHATE | non-polymer | 409.2 | 1 | Chemie (A23) | |||
| 7 | K, L, M, N, O (A, B, I, F, H) | water | water | 18.0 | 349 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 387 (UniProt: C7DIA5)
| PDB | External Database | Details |
|---|---|---|
| Leu 388 | - | expression tag |
| Glu 389 | - | expression tag |
| His 390 | - | expression tag |
| His 391 | - | expression tag |
| His 392 | - | expression tag |
| His 393 | - | expression tag |
| His 394 | - | expression tag |
| His 395 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 99696.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 649.4 | |
| All* | Total formula weight | 100345.7 |
