9W2G

Cryo-EM structure of EB1001-bound neurokinin 2 receptor (NK2R) in complex with miniGs/q70

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
2	B (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	229	26451.0	1		Homo sapiens
3	C (N)	Nanobody 35	polymer	134	14714.3	1		Lama glama
4	D (R)	Substance-K receptor	polymer	406	45419.8	1	UniProt (P21452)	Homo sapiens (human)	SKR,NK-2 receptor,NK-2R,Neurokinin A receptor,Tachykinin receptor 2
5	E (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
6	F (C)	ASP-LYS-TYR-VAL-GLY-MLE-NLE-NH2	polymer	8	820.0	1		synthetic construct

Sequence modifications

R: 1 - 398 (UniProt: P21452)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	134684.3
All*	Total formula weight	134684.3

*Water molecules are not included.