9W0M

Cryo-EM structure of S1P2 in complex with heterotrimeric G protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sphingosine 1-phosphate receptor 2	polymer	405	44592.1	1	UniProt (O95136)	Homo sapiens (human)	S1P receptor 2,S1P2,Endothelial differentiation G-protein coupled receptor 5,Sphingosine 1-phosphate receptor Edg-5,S1P receptor Edg-5
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	54	6000.0	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38745.4	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein

Sequence modifications

A: 1 - 353 (UniProt: O95136)

PDB	External Database	Details
Met -15	-	initiating methionine
Lys -14	-	expression tag
Thr -13	-	expression tag
Ile -12	-	expression tag
Ile -11	-	expression tag
Ala -10	-	expression tag
Leu -9	-	expression tag
Ser -8	-	expression tag
Tyr -7	-	expression tag
Ile -6	-	expression tag
Phe -5	-	expression tag
Cys -4	-	expression tag
Leu -3	-	expression tag
Val -2	-	expression tag
Phe -1	-	expression tag
Ala 0	-	expression tag
Trp 354	-	expression tag
Ser 355	-	expression tag
His 356	-	expression tag
Pro 357	-	expression tag
Gln 358	-	expression tag
Phe 359	-	expression tag
Glu 360	-	expression tag
Lys 361	-	expression tag
Gly 362	-	expression tag
Gly 363	-	expression tag
Gly 364	-	expression tag
Ser 365	-	expression tag
Gly 366	-	expression tag
Gly 367	-	expression tag
Gly 368	-	expression tag
Ser 369	-	expression tag
Gly 370	-	expression tag
Gly 371	-	expression tag
Ser 372	-	expression tag
Ala 373	-	expression tag
Trp 374	-	expression tag
Ser 375	-	expression tag
His 376	-	expression tag
Pro 377	-	expression tag
Gln 378	-	expression tag
Phe 379	-	expression tag
Glu 380	-	expression tag
Lys 381	-	expression tag
Asp 382	-	expression tag
Tyr 383	-	expression tag
Lys 384	-	expression tag
Asp 385	-	expression tag
Asp 386	-	expression tag
Asp 387	-	expression tag
Asp 388	-	expression tag
Lys 389	-	expression tag

C: 9 - 62 (UniProt: P59768)

PDB	External Database	Details
Gln 17	Glu 17	conflict
Gln 58	Glu 58	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Glu 6	Gln 6	conflict
Gln 130	Glu 130	conflict
Asp 237	Asn 237	conflict

D: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	129784.6
All*	Total formula weight	129784.6

*Water molecules are not included.